Anti-APOLIPOPROTEIN A-I APOA1 Antibody

Product Name

Anti-APOLIPOPROTEIN A-I APOA1 Antibody

View all Apolipoprotein A-I/ApoA1 Antibodies

SKU/Catalog Number

A00717-1

Size

1mg

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-APOLIPOPROTEIN A-I APOA1 Antibody (Catalog # A00717-1). Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-APOLIPOPROTEIN A-I APOA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00717-1)

Host

Goat

Contents

0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

apoLipoprotein Type A-I was isolated from human plasma by density gradient centrifugation followed by HPLC purification, followed by repeated immunizations in goat.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00717-1 is reactive to APOA1 in Human

Applications

A00717-1 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

30.778kDa

Background of Apolipoprotein A-I/ApoA1

Anti-Apolipoprotein A-I antibody recognizes the gene product of APOA1. Apolipoprotein promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A-I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids; an 18-amino acid signal peptide is removed co-translationally and a 6-amino acid propeptide is cleaved post-translationally. Variation in the latter step, in addition to modifications leading to so-called isoforms, is responsible for some of the polymorphism observed. APOA1 is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the C-III gene is transcribed convergently in relation to A-I. Defects in the apolipoprotein A-1 gene are associated with HDL deficiency and Tangier disease. Anti-Apolipoprotein A-I is useful for researchers interested in cardiovascular research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:20,000
IHC: 1:50 - 1:200
IF Microscopy: User optimized
IP: 1:100
WB: 1:1,000 - 1:2,000

Validation Images & Assay Conditions

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Coommassie stained gel showing both free and HDL bound apoA-I eluted from a solid phase resin prepared using goat anti-Apolipoprotein A-I antibody (Catalog # A00717-1). The resin was reacted with human serum prior to washing and elution of bound proteins. The gel was composed of 0.75% agarose in a native buffer system. Separation occurred at room temperature.

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Apolipoprotein A-I was detected in paraffin-embedded sections of human liver tissues using goat anti-Apolipoprotein A-I antibody (Catalog # A00717-1) at 5 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1023).

Gene/Protein Information For APOA1 (Source: Uniprot.org, NCBI)

Alternative Names

Alp-1; APOA1; Apo-AI; apoA-I; Apolipoprotein A1; Apolipoprotein AI; Apolipoprotein A-I; Brp-14; Ltw-1; Lvtw-1; MGC117399; Sep-1; Sep-2 APOA1 HPALP2, apo(a) apolipoprotein A1 apolipoprotein A-I|apo-AI|epididymis secretory sperm binding protein

For more info on APOA1, check out the APOA1 Infographic

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In this infographic, you will see the following information for APOA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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